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KMID : 1098420090170010046
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Korean Journal of Medicinal Crop Science
2009 Volume.17 No. 1 p.46 ~ p.53
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Phylogenetic Analysis of Schizonepeta Spike on the Basis of DNA Sequences
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Jigden Baigalmaa
Kim Myung-Kyum
Noh Chong-Hoon
Sun Hua
Yang Deok-Chun
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Abstract
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Schizonepeta spike (Korean name "Hyung-Gae") has been used for oriental medicinal purposes in Korea, China and Japan. In this study, twenty six "Hyung-Gae" samples were collected including nine certified Schizonepeta tenuifolia plants, and seventeen commercially marketed "Hyung-Gae" products. Chloroplast trnL-F and rDNA ITS regions of the "Hyung-Gae" samples were sequenced and used to identify whether the samples were genuine S. tenuifolia or not. As the result, the trnL-F and ITS sequences of all the "Hyung-Gae" samples were shown to be identical and it was proven that commercially available medicinal products "Hyung-Gae" are genuine S. tenuifolia. Phylogenetic tree of S. tenuifolia using the trnL-F sequences was constructed and compared with phylogenetic tree using ITS of rDNA region sequences. In these tree, S. tenuifolia was affiliated in the family Lamiaceae. It is proven that trnL-F and ITS phylogenetic trees are useful to study taxonomic position of S. tenuifolia.
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KEYWORD
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Hyung-Gae (Schizonepeta tenuifolia), Phylogenetic Analysis, trnL-F, ITS
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